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中国精品科技期刊2020
易湘茜, 李家怡, 高程海, 张卿, 周文红, 邓家刚. 无瓣海桑果实乙醇提取物及其不同极性萃取物抗氧化活性[J]. 华体会体育, 2017, (19): 27-30. DOI: 10.13386/j.issn1002-0306.2017.19.005
引用本文: 易湘茜, 李家怡, 高程海, 张卿, 周文红, 邓家刚. 无瓣海桑果实乙醇提取物及其不同极性萃取物抗氧化活性[J]. 华体会体育, 2017, (19): 27-30. DOI: 10.13386/j.issn1002-0306.2017.19.005
YI Xiang-xi, LI Jia-yi, GAO Cheng-hai, ZHANG Qing, ZHOU Wen-hong, DENG Jia-gang. Antioxidant activity of alcohol extract and different polar parts of Sonneratia apetala fruits[J]. Science and Technology of Food Industry, 2017, (19): 27-30. DOI: 10.13386/j.issn1002-0306.2017.19.005
Citation: YI Xiang-xi, LI Jia-yi, GAO Cheng-hai, ZHANG Qing, ZHOU Wen-hong, DENG Jia-gang. Antioxidant activity of alcohol extract and different polar parts of Sonneratia apetala fruits[J]. Science and Technology of Food Industry, 2017, (19): 27-30. DOI: 10.13386/j.issn1002-0306.2017.19.005

无瓣海桑果实乙醇提取物及其不同极性萃取物抗氧化活性

Antioxidant activity of alcohol extract and different polar parts of Sonneratia apetala fruits

  • 摘要: 研究了无瓣海桑果实乙醇提取物及其不同极性萃取物的抗氧化活性。无瓣海桑果实采用95%乙醇浸提后经减压浓缩得到乙醇提取物,然后依次采用乙酸乙酯、正丁醇等有机溶剂进行萃取并收集剩余液体得到乙酸乙酯相萃取物、正丁醇相萃取物、水相残留物。在超氧阴离子自由基、DPPH自由基、ABTS+自由基清除能力和还原能力的抗氧化活性测试体系中,无瓣海桑果实乙醇提物及不同极性萃取物均具有一定的抗氧化活性,并且呈明显的量效关系。乙醇提取物在DPPH自由基清除和还原能力测定中表现出较好的抗氧化活性。乙酸乙酯相萃取物在4种测定体系中均表现出很好的抗氧化活性,且其DPPH自由基清除活性(IC50=1.69μg·m L-1)明显强于阳性对照VE(IC50=6.06μg·m L-1),超氧阴离子自由基清除活力(IC50=0.35 mg·m L-1)与阳性对照VC(IC50=0.30 mg·m L-1)相近。 

     

    Abstract: The antioxidant activities of alcohol extract and different polar parts of Sonneratia apetala fruits were studied. The ethanol extract was obtained by the addition of ethanol, and then successively extract by ethyl acetateand, butanol and the remaining liquid was collected to obtain n-butanol phase, ethyl acetate phase and aqueous phase residue. The antioxidant activity of all extracts were evaluated by superoxide anion radical, DPPH free radical, ABTS+free radical scavenging ability and reducing ability. The results showed that all test samples had antioxidant activities and showed significant dose-effect relationship.Alcohol extraction showed medium antioxidant activity in the determination of DPPH radical scavenging and reducing ability. The ethyl acetate phase exhibited strong antioxidant activity in DPPH free radical, ABTS+free radical scavenging and reducing ability, its DPPH free radical scavenging activity ( IC50= 1.69 μg·m L-1) was obviously stronger than positive control vitamin E ( IC50= 6.06 μg·m L-1) , and its superoxide anion radical scavenging activity ( IC50= 0.35 mg·m L-1) showed no difference from control ( IC50= 0.30 mg·m L-1) .

     

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