Abstract: With liquid chromatography-mass spectrometry technology developing continuously, the liquid chromatography-mass spectrometry technology has been widely available in the field of medicine, environment, food security recently, and also play a key role in plant protein and peptide research.Recently advances on development of liquid chromatography-mass spectrometry and liquid chromatography-mass spectrometry application in the cereals, legumes and other crop proteins were reviewed. The development trend and prospects of plant protein research by liquid chromatography-mass spectrometry were used to provide the latest basic theory for the further study of plant proteins.