Abstract: In this paper, the preparation process of oyster bioactive peptides was optimized by response surface method. The effect of Na Cl concentration, liquid-solid ratio, temperature and extraction time on the scavenging rate of hydroxyl radicals was investigated.The result showed that optimum extraction conditions were: Na Cl concentration 0.09%, liquid-solid rate 57 m L/g, time 2.5 h.Under this condition, the predictive value clearance rate of hydroxyl radical was 33.09% and the measured value was33.34%, and the relative error was only 0.76%.It showed that the optimum extracting condition of response surface method was reasonable and feasible. The best result of bioactive peptides antioxidant activity indicated that it had a relatively good scavenging ability to hydroxyl radical, DPPH free radical and H2O2. Meanwhile, it also had reducing power and ferrous ion chelating capacity. On the whole, Onchidium struma bioactive peptides reflect a certain antioxidant activity and has a good prospect of development.