海参体壁蛋白ACE抑制肽酶解工艺及产物分子量分布研究

Study on hydrolysis condition and molecular weight distribution of ACE inhibitory peptide derived from sea cucumber protein

摘要: 以血管紧张素转化酶(ACE)抑制率和水解度为指标,筛选出适合海参体壁蛋白水解的酶。采用单因素和响应面法优化最优酶的水解条件,确定其最佳水解工艺,进而采用HPLC法探讨酶解产物的分子量分布。结果表明:中性蛋白酶在50℃、p H7.5、酶量3000 U/g、酶解5 h条件下,所得酶解产物的ACE抑制率最高,达到56.5%。此条件下酶解产物经HPLC分析,200~1000 Da(2~10肽)的海参多肽占44.1%。

Abstract: With the hydrolysis degree and ACE inhibitory rate the index, the optimum enzyme was selected for hydrolyzing sea cucumber protein. The optimal condition for enzymatic hydrolysis was investigated through single factor experiment and orthogonal.HPLC was used to determine the molecular distribution of the hydrolysate. The results showed that the optimum enzyme was neutral protease.The optimized parameters were temperature 50 ℃, time 5h, p H7.5 and amount of enzyme 3000U/g.Under this condition, ACE inhibition rate was up to 56.5% and the relative molecular weight of 200¹000 Da ( 2¹0 peptides) reached 44.1%.