钝裂银莲花居群遗传多样性及其与生态因子相关性
Genetic diversity in Anemone obtusiloba populations and its relationship with ecological factors
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摘要: 采用RAPD技术分析了4个地区(合作、碌曲、玛曲、阿孜)不同花色(黄、浅、白)11个钝裂银莲花居群的遗传结构。结果表明:钝裂银莲花居群遗传多样性较高,Shannon多样性指数(I)为0.6025,Nei’s基因多样性指数(H)为0.4166,Nei’s基因分化系数(Gst)和AMOVA分析表明钝裂银莲花居群间分化程度较低,大部分变异存在于居群内(67%),居群间占一小部分(33%)。对不同居群进行UPGMA聚类分析,结果显示11个亚居群可以归为3大支。相关性分析表明遗传多样性与地理分布和土壤营养状况有相关性。Abstract: The genetic structure of 11 different populations of floral-color Anemone obtusiloba in 4 different areas of Gannan were evaluated using random amplified polymorphism DNA ( RAPD) .The results showed that the genetic diversity in Anemone obtusiloba populations was quite high: The Shannon index ( I) was 0.6025 and Nei's gene diversity ( H) was 0.4166. Moreover, the differentiation level among populations was low based on Nei's Gst value and analysis of molecular variance ( AMOVA) .Less than 33% of genetic diversity was attributed to the differentiation among populations while more than 67% resided within the different populations.According to unweighted pair group method using arithmetic averages ( UPGMA) result, 11 populations of A.obtusiloba were clustered into 3 groups. Further correlation analysis showed that genetic diversity and distance correlated with geographical distribution and soil nutrition.
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