Abstract: Grape is vulnerable to various external abiotic factors during grape berry development.Proteomics is a powerful tool to study proteins from large scale. The application of proteomics in grape research will help to clarify the physiological and metabolic mechanism under abiotic stresses. In this paper, the latest researches progress of proteomics in grapes during grape berry development and under major abiotic stresses ( water deficit, temperature stress and light stress) were reviewed. The potential problems in the research field were discussed, and the research emphasis in the future was suggested, in order to provide reference for the future studies on plant development physiology and stress physiology, and provide theoretical basis for agricultural production and crop quality improvement.