Abstract: In the detection of triazine herbicides, the development of immunoassay has gotten more and more social attention and it has been the research hotspots. In this study, several key steps of immunoassay on the determination of triazine herbicides were introduced.These steps contained design and synthesis of hapten and artificial antigen, preparation of antibody and the application of many kinds of immunoassay on the detection of triazine herbicides.Besides, the current problems and the prospect of the field were also discussed.