Abstract: Response surface methodology was employed for the ultrasonic-assisted extraction of anthocyanins from Lycium ruthenicu Murr.Anthocyanin yield was selected as evaluation index to investigate anthocyanin extraction process from Lycium ruthenicu Murr.On the basis of one-factor-at-a-time experiments, response surface methodology was used to optimize the extraction process of anthocyanins from Lycium ruthenicu Murr with four significant impact factors such as ethanol concentration, liquid-to-solid ratio, extraction time, extraction temperature. The results showed that the optimum conditions were as follows: 72% of ethanol concentration, 27 ∶ 1 ( m L/g) of liquid-to-solid ratio, 16 min of extraction time, 38 ℃ of extraction temperature. Under these conditions, the actual average yield was ( 9.16 ± 0.059) mg/g, relative error of 0.43%compared with the predicted value and incresed nearly 1-fold compared with that obtained without ultrasonic treatment.