Abstract: Response surface methodology was used to optimize the ultrasonic assisted extraction conditions of anthocyanin from purple corn cob and improve the extraction rate.Anthocyanin freeze-dried powder mixed with maltodextrin was processed into powder by spray drying method. The results showed that the optimum conditions were as follows: the solid-liquid ratio, extraction temperature, extraction time and ultrasonic time were 1 ∶ 13 ( g/m L) , 41 ℃, 31 min and 20 min, respectively. The anthocyanin content and the extraction yield were ( 4.623 ± 0.021) mg/g and 94.8% at this condition. The anthocuanin powder had low moisture 4.8% and embedding rate 86.7% when the inlet air temperature, the mass ratio ( anthocyanin∶ maltodextrin) and the solid concentration during spray-drying were 150 ℃, 1 ∶ 1 and 15%, respectively. The conditions of anthocyanin extraction from purple corn cob and anthocyanin powder preparation were optimized by the single factor experiment and the response surface methodology, and the results provided a reference for the development of purple corn cob.