Abstract: Firstly, the effect of ultrasonic power, extracting temperature and the solid-liquid ratio on oil yield of Acer truncatum Bunge oil was established. The appropriate temperature of artificial germination of seeds was also investigated. The content of nervonic acid in the seeds oil was detected by GC analysis. The effect of the seed oil on the proliferation of nerve cells was detected by MTT.As the results shown, the optimal extraction conditions were as follows: ultrasonic power 200 W, extracting temperature 25 ℃, the solid-liquid ratio 1∶ 12.The seeds of Acer truncatum Bunge were germinated at 4 ℃. The results of GC analysis showed that the content of nervonic acid was significantly increased to 1.5 fold after the germination. Nervonic acid could promote the growth of nerve cells, and the oil from the germinated seeds of Acer truncatum Bunge was stronger than the dormant seed oil.