Abstract: In this paper, the technology of anti-browning of Agaricus bisporus was optimized by response surface methodology.According to the effects of single factor test, four main factors about citric acid, disodium stannous citrate ( DSC) , sodium erythorbate, L-cysteine were variables, and Agaricus bisporus browning degree was response value, the experimental design method of Box-Benhnken was used to study the effects of each variable and their interactions on the Agaricus bisporus browning degree.The prediction model of regression equation by using Design Expert software was analyzed by response surface analysis.The result showed that the optimum conditions of anti-browning were that mass fraction of citric acid 0.97%, citric acid stannous disodium 0.05%, L-cysteine 0.57%, sodium erythorbate 0.53%.Under this condition, the prediction value of Agaricus bisporus browning degree was 2.52 and the color proction effect was good.