细胞外膜蛋白酶编码基因prtP对干酪乳杆菌粘附性的影响
Effect of extracellular membrane protease gene prt P on the adhesion of Lactobacillus casei
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摘要: 目的:探究prt P基因与干酪乳杆菌（21858）粘附的相关性。方法:利用λ-Red同源重组方法,构建21858 prt P基因缺失突变株,并测定亲本株和突变株的生长曲线、疏水性、自凝聚率、对小肠粘液粘附率及细胞粘附性。结果:亲本株与突变株生长速度相似,突变株疏水率为44.1%,大于亲本株的23.9%;突变株自凝聚率和对肠粘液的粘附率在各时间点均小于亲本株,对细胞的粘附性也小于亲本株。结论:prt P基因与干酪乳杆菌21858粘附性相关,是决定干酪乳杆菌粘附性的重要因子。Abstract: Objective: To explore the role of the prt P gene of Lactobacillus casei ( 21858) in it's adhesion. Methods: Using red homologous recombination method to build 21858 prt P gene deletion mutant was built.And the growth curve, hydrophobic, auto-aggregation, the rate of intestinal mucus adhesion and the adhesion of cells were measured. Results: The growth curve was similar to the mutant strains.The hydrophobic rate of mutant strains was 44.1% greater than 23.9% of the parent strains. The auto-aggregation rate of mutant strains at each time points were less than the parent plants, and intestinal mucus adhesion rate of mutant strains was less than the parent plants.The adhesion of mutant strains to both cells were less than the parent plants.Conclusion: The prt P genes associated with Lactobacillus casei 21858 adhesive, is an important factor in determining the adhesion of lactic acid bacteria.
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