椰子谷蛋白-1功能特性的分析
Analysis on functional properties of coconut glutelin-1
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摘要: 本实验采用分步提取和膜分离结合的方法从椰肉中提取椰子谷蛋白-1,测定其氨基酸组成,利用SDS-PAGE和柱层析技术分析其分子量分布和亚基组成,利用DSC技术分析其耐热性,然后对谷蛋白-1的乳化性和起泡性进行了分析。结果表明:椰子谷蛋白-1中含有16种氨基酸,天冬氨酸和精氨酸的含量很高,SDS-PAGE表明,椰子谷蛋白-1含有5个亚基,分子量分布在19.650.6 k Da之间。DSC扫描结果表明,椰子谷蛋白-1具有较好的热稳定性。功能特性实验表明,椰子谷蛋白-1有良好的乳化稳定性(85.17%±1.41%)和泡沫稳定性(89.15%±5.75%),均优于大豆分离蛋白。该结果表明椰子谷蛋白-1具有开发为乳化稳定剂和泡沫稳定剂的潜力。Abstract: The glutelin-1 was extracted from coconut with step by step method assisted with membrane purification technology.Then the amino acid composition, distribution of molecular weight and subunits, thermal property, emulsifying and foaming properties of coconut glutelin1 were studied. Results showed that the glutlelin-1 contained 16 kind of amino acid, with the feature of a large amount of glutamic acid and arginine. There were five subunits with molecular weight 19.6 to 50.6 k Da in coconut glutelin-1.Moreover, coconut gultelin-1 exhibited good emulsion stability ( 85.17% ± 1.41%) and foaming capacity and stability ( 89.15% ± 5.75%) , which was all higher than that of soybean protein isolate.This meant that coconut gultelin-1 had potential usage in food industry as emulsion stabilizer and foam stabilizer.