Abstract: Objective: To investigate the effect of glycyrrhiza selenium polysaccharide on acute liver injury induced by CCl₄ in mice.Methods: TP, ALB, ALT, AST, ALP, γ-GT levels in serum and GPX, MDA levels in liver tissues were examined. In addition, pathologic changes were examined by HE staining. Results: Glycyrrhiza selenium polysaccharide of middle and high dose can reduce the degrees of AST ( p < 0.05) , ALP ( p < 0.05) in serum and MDA and GSH-Px ( p < 0.05) levels in liver tissue, and the ALT level in serum were decreased in high dose group, which can improve CCl₄-induced pathological tissue damage.Conclusion: This study confirms licorice selenium polysaccharide on CCl₄-induced acute liver injury has protective effect, the mechanism is likely to be reached by scavenging free radicals and prevent liver damage.