Abstract: In order to study the growth, reproduction and control of sweet cherry, different concentrations of Nisin solution, citric acid solution and mixed solutions were used to handle sweet cherry inoculated with Salmonella, to observe and analysis the inhibitory effect of Nisin and citric acid. The results showed that both Nisin solution and citric acid solution could achieve antibacterial effect, and with the increase of Nisin ( 10 μg/m L to 100 μg/m L) and citric acid ( 1 g/L to 5 g/L) , the inhibitory effect increased significantly.At the same time, the results also showed that antibacterial effect of 100 μg/m L Nisin solution and5 g/L citric acid solution, 10 μg/m L Nisin and 3 g/L citric acid mixed solution and 50 μg/m L Nisin and 1 g/L citric acid mixed solution was basically the same, colony concentration can be reduced to 104cfu/m L after treatment for 5 min, when using50 μg/m L Nisin and 3 g/L citric acid mixed solution for 15 min to handle samples, the concentration of Salmonella colonies decreased to below the detection limit, with the increase of storage time to tenth day, Nisin and citric acid remain antibacterial effects as usual, fully achieved the antibacterial sterilization effect.