SPME和SDE结合GC-MS分析荷叶中挥发性成分
Characterisation of volatile components of Lotus leaves by HS-SPME and SDE coupled to GC-MS
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摘要: 分别采用同时蒸馏萃取（SDE）法和固相微萃取（SPME）法并结合气相色谱-质谱联用（GC-MS）技术分析荷叶的挥发性风味成分。SPME法选用3种萃取头（CAR/PDMS、DVB/CAR/PDMS、PDMS/DVB）与SDE法对定性结果进行比较分析。结果表明,SDE法所鉴定出的挥发性成分有97种,相对含量为94.86%,且以烃类物质为主;SPME（CAR/PDMS）法所鉴定出的挥发性成分有29种,相对含量为89.26%,以醇类为主;SPME（DVB/CAR/PDMS）法所鉴定出的挥发性成分有62种,相对含量为85.97%,以醛类为主;SPME（PDMS/DVB）法所鉴定出的挥发性成分有59种,相对含量为86.96%,以醛类为主。由此可见,在荷叶挥发性成分分析中,四种方法萃取到的挥发性成份种类数从多到少依次是,SDE、SPME（DVB/CAR/PDMS）、SPME（PDMS/DVB）、SPME（CAR/PDMS）。Abstract: The volatile flavor components of lotus leaves were extracted by solid phase microextraction ( SPME) , simultaneous distillation extraction ( SDE) , which was analyzed by gas chromatography-mass spectrometry ( GC-MS) .Solid phase microextraction ( SPME) of three extraction heads ( CAR/PDMS, DVB/CAR/PDMS, PDMS/DVB) and simultaneous distillation extraction ( SDE) were used to compare qualitative results. The results showed that there were 97 kinds of volatile components identified by SDE and the percent of total peak area was 94.86% and the major compound was alkane, there were 29 kinds of volatile components identified by SPME ( CAR/PDMS) and the percent of total peak area was 89.26% and the major compound was ketone, there were 62 kinds of volatile components identified by SPME ( DVB/CAR/PDMS) and the percent of total peak area was 85.97% and the major compound was aldehyde, there were 59 kinds of volatile components identified by SPME ( PDMS/DVB) and the percent of total peak area was 86.96% and the major compound was aldehyde.This showed that the most kind of Volatile components can be obtained by SDE, followed by SPME ( DVB/CAR/PDMS) 、SPME ( PDMS/DVB) 、SPME ( CAR/PDMS) .
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