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中国精品科技期刊2020
符群, 赵红华, 刘峰, 任红波, 魏俊青, 陈国峰. 超高效液相色谱-质谱联用法测定山野菜中5种农药的残留[J]. 华体会体育, 2017, (15): 238-243. DOI: 10.13386/j.issn1002-0306.2017.15.044
引用本文: 符群, 赵红华, 刘峰, 任红波, 魏俊青, 陈国峰. 超高效液相色谱-质谱联用法测定山野菜中5种农药的残留[J]. 华体会体育, 2017, (15): 238-243. DOI: 10.13386/j.issn1002-0306.2017.15.044
FU Qun, ZHAO Hong-hua, LIU Feng, REN Hong-bo, WEI Jun-qing, CHEN Guo-feng. Determination of five pesticides residues in wild vegetables by ultra performance liquid chromatography-tandem mass spectrometry[J]. Science and Technology of Food Industry, 2017, (15): 238-243. DOI: 10.13386/j.issn1002-0306.2017.15.044
Citation: FU Qun, ZHAO Hong-hua, LIU Feng, REN Hong-bo, WEI Jun-qing, CHEN Guo-feng. Determination of five pesticides residues in wild vegetables by ultra performance liquid chromatography-tandem mass spectrometry[J]. Science and Technology of Food Industry, 2017, (15): 238-243. DOI: 10.13386/j.issn1002-0306.2017.15.044

超高效液相色谱-质谱联用法测定山野菜中5种农药的残留

Determination of five pesticides residues in wild vegetables by ultra performance liquid chromatography-tandem mass spectrometry

  • 摘要: 建立超高效液相色谱-质谱联用法ACQUITY UPLC/Xevo TQ-S测定人工栽培山野菜中啶虫脒、吡虫啉、多菌灵、嘧霉胺、苯醚甲环唑5种农药残留。以乙腈为提取溶剂,采用多重反应离子监测(MRM)模式,ACQUITY UPLC BEH C18为分析色谱柱、0.1%甲酸乙酸铵-乙腈为流动相,对样品进行检测分析。结果表明:5种农药在0.00052.0000 mg/L范围内与峰面积呈良好线性关系,相关系数在0.99470.9999之间。在农药混合标准溶液0.0050.200 mg/kg的水平下,添加回收率均在73.43%115.84%之间,相对标准偏差为2.04%18.19%。检出限在0.0200.510μg/kg范围内;定量限在0.0661.699μg/kg范围内。本方法操作简单、快捷、准确度和精密度高,可应用于山野菜中5种农药残留测定。 

     

    Abstract: A method was established for determining acetamiprid, imidacloprid, carbendazim, pyrimethanil, difenoconazole residues in wild vegetables by ultra-high performance liquid chromatography-mass spectrometry ( ACQUITY UPLC/Xevo TQ-S) . Residues in samples were extracted with acetonitrile, and separated by C18 column with gradient elution using a mobile phase made up of acetonitrile and 0.1% formic acid ammonium acetate and detected. The results showed the linear range of0.00052.0000 mg/L with the correlation coefficients from 0.9947 to 0.9999.The recoveries rates at 0.0050.200 mg/kg levels were in the range of 73.43% 115.84%.The relative standard deviation ( RSD) was 2.04% 18.19%.The limits of detection were0.0200.510 μg/kg and the limits of quantitation were 0.0661.699 μg/kg.The method proved to be simple, sensitive, rapid, accurate and precise for the determination of five kinds of pesticide residues in wild vegetables.

     

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