Halobacterium salinarium 5 L发酵罐中产细菌视紫红质(BR)发酵条件优化

Fermentation conditions optimization of producing bacteriorhodopsin by Halobacterium salinarium in a 5 L fermentor

摘要: 优化盐沼盐杆菌（Halobacterium salinarium）在5 L发酵罐中的生物量及细菌视紫红质含量的发酵条件,为该菌株的进一步应用提供依据。通过单因素实验考察温度、p H、搅拌转速、通气量和不同流速的玉米浆对盐沼盐杆菌的影响。优化后发酵条件为:温度37℃,转速250 r/min,通气量1.3 vvm,p H在发酵的过程中不用酸液和碱液调节,补加玉米浆的流速为0.2 m L/min。在此条件下,菌体的生物量(用OD₆₀₀表示)达到4.531,细菌视紫红质的含量达到90.86 mg/L,与未优化的条件相比分别提高了43.8%、36%。

Abstract: The technological conditions for bacteriorhodopsin and biomass by the Halobacterium salinarium in a 5 L fermenter were optimized, in order to provide the basis for further application of the strain.The effects of temperature, p H, agitation speed, ventilation volume and corn pulp with different flow rates on the Halobacterium salinarium were investigated by single factor experiments.The optimum fermentation conditions were temperature at 37 ℃, agitation speed at 250 r/min, ventilation volume at 1.3 vvm, flow rate of corn pulp at 0.2 m L/min. p H was not regulated by acid and alkali in the fermentation process. Under these conditions, the maximum biomass was 4.531, the maximum BR yield was 90.86 mg/L, which was about respectively43.8% 、36% greater than those obtained with initial fermentation conditions.