Abstract: In this article, the phage display technology was described, and its application in food safety was summarized. With the development of the phage display technology, it is widely used in the food safety. It can be used as an efficient method of generation of antibody to detect antibiotic, bio-toxin and small molecule in food, used to screening mimic for developing green detection technology, and the control of foodborne pathogenic bacterial. In addition, this technology also showed broad application in the field of developing novel food preservatives.