响应面法优化坛紫菜中镉的脱除工艺及其营养品质变化
Optimization of cadmium removing from Pyropia haitanensis and its change of nutritional quality by response surface methodology
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摘要: 探究超声辅助柠檬酸对坛紫菜中镉脱除的最佳工艺条件,并评价其对坛紫菜营养品质的影响。通过单因素实验研究柠檬酸浓度、超声功率和超声时间对坛紫菜中镉脱除率的影响,进而以镉脱除率和坛紫菜色差为响应指标,利用Box-Behnken实验对以上因素进行响应面优化,并分析脱除前后坛紫菜的营养品质变化。结果表明,坛紫菜中镉的最佳脱除工艺为柠檬酸浓度0.06 mol/L、超声功率220 W和超声时间20 min,此时,镉的脱除率为98.5%,藻体脱除前后的色差为3.149。应用该工艺条件处理,坛紫菜的基本营养成分未受到显著影响,蛋白质和脂肪酸仍保持较好的营养质量。该脱除工艺操作简单,镉脱除效果好,且不影响坛紫菜的营养品质,为工业生产中解决坛紫菜的镉脱除问题提供思路。Abstract: The objectives of this study were to develop an optimized method for removing cadmium from Pyropia haitanensis and to evaluate the nutritional value. Response surface methodology ( RSM) based on a Box-Behnken design was used in combination with single factor experiments to establish the optimal removal conditions as follows: citric acid concentration 0.06mol/L, ultrasonic power 220 W, ultrasonic treatment time 20 min, contributing to a removal rate of 98.5% and chromatic aberration of 3.149.Under this condition, the basic nutrient component, amino acids and fatty acids hadn't been affected. This removal procedure was simple and the obtained products had good quality with low cadmium level, so that it could be used in industrial production of Pyropia haitanensis.