解淀粉芽孢杆菌HRH317抗菌蛋白发酵条件优化及其稳定性研究

Optimization and stabilization of antifungal protein from Bacillus amyloliquefaciens strain HRH317

摘要: 本文以抑菌圈直径为指标,采用单因素实验和响应面法对解淀粉芽孢杆菌HRH317菌株产抗菌蛋白的发酵条件进行优化。得到最佳发酵条件为:初始p H为7.0的NB培养液,接种量4%,37℃下振荡培养24 h,转速为200 r/min。在此条件下抗菌蛋白的抑菌圈直径达21.85 mm,结果表明该抗菌蛋白具有良好的抑菌效果。稳定性研究结果表明:蛋白在40~80℃、p H2~11稳定,对蛋白酶K有一定耐受性。

Abstract: This paper is concerned with the antifungal protein extraction from the strains Bacillus amyloliquefaciens HRH317.The inhibitive circle diameter as evaluating indicator, the fermentation condition were optimized by single factor experiments and response surface methodology ( RSM) .The results showed the optimized extraction parameters were that the initial p H value of nutrient broth medium ( NB) was 7, inoculation volume of 4%, 37 ℃, and the shaker rotation speed 200 r/min.The inhibitive circle diameter was up to 21.85 mm.The results indicated that the antifungal protein was proved to have strong inhibition effect.The antifungal protein was tested to have strong tolerant to proteinase K, which could be steady with 40~80 ℃ and p H2~11.