响应面法优化苋红素提取工艺及其抗氧化性

熊茜; 王春幸; 孙朦; 王凯凯; 宋佳敏; 许凤; 王鸿飞

doi:10.13386/j.issn1002-0306.2017.12.040

响应面法优化苋红素提取工艺及其抗氧化性

Response surface methodology for optimizing extraction of amaranthin and its antioxidant activity

摘要

摘要: 以彩苋为原料,通过单因素实验,研究提取溶剂、提取时间、提取温度、料液比对苋红素得率的影响,利用响应面法对苋红素得率进行优化,得到最优工艺条件。并对提取出的苋红素粗提液进行抗氧化性研究。结果表明,苋红素得率的最佳提取工艺条件为H₂O作为提取剂,料液比为1∶50（m∶v,g/m L）,提取温度为30℃,提取时间为44 min。在此最优条件下,模型预测提取出的苋红素得率为0.308%,验证实验得到的苋红素得率为0.305%,两者误差为0.98%,证明此工艺条件合理可行。另外,苋红素具有较好的抗氧化能力,对1,1-二硝基苦基苯肼（1,1-diphenyl-2-picrylhydrazyl,DPPH）自由基、羟基自由基（·OH）、超氧阴离子自由基（O₂^-·）的IC₅₀分别为0.18、0.06和0.35 mg/g。

Abstract: The appropriate extraction conditions of amaranthin (extractant, extraction time, extraction temperature and solid-liquid ratio) from Amaranthus tricolor L.by single factor experiment were determined.Response surface methodology was applied to optimize the extraction process, and then the antioxidant activity of amaranthin crude extract was studied.The results showed that the optimal conditions were as follows: solid-liquid ratio 1∶ 50 (m∶ v, g/m L) , extraction temperature 30 ℃ and extraction time 44 min with H₂O as extractant. Under these conditions, the predicted content of amaranthin was 0.308% with a relieve error of 0.98% when compared to the actual content, 0.305%. Thus, the optimized extraction conditions were reliable. In addition, there were obvious antioxidant activities in amaranthin, which the IC₅₀ value of DPPH, ·OH and O₂^-· free radical scavenging abilities were 0.18, 0.06, 0.35 mg/g, respectively.