Abstract: The experiment prepared palm olein-based special baking release agent, using the palm olein as base material and Carnauba Wax as auxiliary material and stabilizing with various additives. By single factor experiment and central composite design response surface methodology, the multiple regression models of emulsification separation ratio and the viscosity of palm olein-based special baking release agent were established to conduct the optimal preparation conditions for the release agent.The results showed that the best conditions were the content of palm wax 5.03%, the dose of lecithin high potency 2.0%, the proportion of PGPR- (Glyceryl Monosterate) 2 ∶ 3, the content of PGPR- (Glyceryl Monosterate) 1.0%, the proportion of composite stabilizer (Xanthan gum, Guar gum and Carrageenan) 1 ∶ 2.5 ∶ 1, the dose of composite stabilizer 0.30%, temperature90 ℃, ultrasonic time 19.75 min and ultrasonic power 110 W, respectively.The emulsification separation ratio and the viscosity of this baking mold release agent were 2.15% and 419.6 m Pa·s, respectively, the cake adhesion rate was 0.367%.In the study of mold release activity, the cake adhesion rate reduced with the increase of the concentration of release agent. The effect of mold release was remarkable.