草鱼鱼皮不同分子量肽段体外抗氧化性能的研究

蔡路昀; 冷利萍; 李秀霞; 吕艳芳; 励建荣; 赵葳; 马永钧; 沈琳

doi:10.13386/j.issn1002-0306.2017.12.011

草鱼鱼皮不同分子量肽段体外抗氧化性能的研究

Evaluation of the in vitro antioxidant properties of different molecular weight peptide fractions from grass carp ( Ctenopharyngodon idella) skin

摘要

摘要: 采用碱性蛋白酶对草鱼皮进行水解,并将水解产物通过截留分子量为10、5和3 k Da的超滤膜,得到>10、5¹0、3⁵和<3 k Da四种不同分子量肽段组分。主要研究了粗蛋白水解产物经截留后不同分子量肽段的体外抗氧化活性及其功能,结果表明相同浓度下3⁵和<3 k Da肽段组分在清除1,1-二苯基-2-三硝基苯肼（DPPH）和2,2’-联氮-二（3-乙基-苯并噻唑-6-磺酸）二铵盐（ABTS）自由基方面比蛋白水解产物或其它肽段组分的效果显著（p<0.05）,而>10 k Da肽段组分与Fe2+螯合能力最强。然而,与还原型的L-谷胱甘肽相比,>10、5¹0、3⁵ k Da肽段组分具有较弱的清除超氧阴离子自由基能力,肽的氨基酸序列在其抗氧化活性中起到关键作用。结果表明,<3 k Da肽段组分可作为功能性天然抗氧化剂添加到保健食品中。

Abstract: Grass carp skins were hydrolyzed using alcalase to obtain crude protein hydrolysate, which was then fractionated by molecular mass into four fractions-> 10, 5~10, 3~5, and < 3 k Da.In vitro antioxidant activities of the crude hydrolysate and its fractions were studied.Results indicated that the 3~5 and < 3 k Da fractions were significantly (p < 0.05) higher in scavenging 1, 1-Diphenyl-2-picrylhydrazyl (DPPH) and 2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt radicals (ABTS) than crude hydrolysate or other fractions, while the > 10 k Da fraction had the highest Fe2 +chelating activity.However, the > 10, 5 ~ 10, and 3 ~ 5 k Da fractions were weaker superoxide radical scavengers compared with reduced L-glutathione. The amino acid sequences of the peptide fractions might play a crucial role in their antioxidant activities.These results indicate that the < 3 k Da fraction has the potential to be used as a natural antioxidant in health food.