Abstract: Anthocyanins were extracted by ethanol from blueberry pomace. The anthocyanin compositions in blueberry pomace were isolated using high performance liquid chromatography ( HPLC) .Oxford cup assay was performed to determine the antibacterial effects of anthocyanins using Escherichia coli, Staphylococcus aureus and Bacillus subtilis as the representatives of the three types of bacteria.The antibacterial spectrum of blueberry pomace-derived anthocyanins was preliminarily identified, and the minimum inhibitory concentration was confirmed by sequential 2-fold dilution. Anti-proliferation activity of anthocyanins at different concentrations against colorectal cancer, gastric cancer and hepatic carcinoma cells were determined by MTT assay.Anthocyanins inhibited the three types of bacteria, with MIC values of 0.63, 0.63, 1.25 mg/m L, for Bacillus subtilis, Staphylococcus aureus and Escherichia coli, respectively.Blueberry pomace-derived anthocyanins also inhibited all three types of cancer cells, with IC50 values of 7.54, 13.34, 21.05 μg/m L for colorectal cancer, gastric cancer, and liver cancer cells, respectively. Blueberry pomace derived anthocyanins have certain antibacterial and anticancer activity, and the study has important theoretical and practical significance to reveal mechanism of developing new anticancer drugs.