响应面法优化超声辅助离子液体提取籽瓜果胶工艺的研究
Optimization of sonication-assisted extraction of pectin from seed melon by response surface methodology
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摘要: 为了探究响应面法对提取的籽瓜果胶半乳糖醛酸含量的影响,以离子液体为提取剂,采用超声技术辅助提取籽瓜中的果胶,对超声功率、超声时间、提取温度、料液比等因素进行响应面实验设计,以果胶提取液中半乳糖醛酸的含量的高低,作为评价果胶的标准。实验结果表明,提取工艺条件为1 mol/L的1-丁基-3-甲基咪唑氯盐（BmimCl）水溶液为提取剂、超声功率300 W、超声时间40 min、提取温度70℃、料液比为1∶20（g/m L）。提取果胶中半乳糖醛酸含量理论值为82.42%,实测值为82.88%,实际测定值与理论计算值基本吻合。该工艺为籽瓜资源的综合利用提供理论基础。Abstract: In order to explore the effect of response surface methodology on the extraction purity of seed pectin, the extraction of pectin from seed water melon by ionic liquid was studied with ultrasonic extraction. The effects of ultrasonic power, ultrasonic time, extraction temperature on the content of galacturonic acid in pectin extract were investigated by response surface design.The optimum conditions were as follows: 1 mol/L 1-butyl-3-methylimidazolium chloride ( BmimCl) as extraction agent, ultrasonic power of 300 W, ultrasonic time of 40 min, extraction temperature of 70 ℃.And the ratio of material to liquid was1∶ 20 ( g/m L) . The theoretical value of galacturonic acid content in extracted pectin was 82.42% and the measured value was82.89%.The actual measured value was in accordance with the theoretical calculation.And this research provide the theoretical basis for comprehensive utilization of seed watermelon resources.
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