响应面法优化多汁乳菇多糖提取工艺及抗氧化活性研究

黄娟; 黄燕燕; 刘冬梅; 陈素芹; 潘伟才

doi:10.13386/j.issn1002-0306.2017.11.002

响应面法优化多汁乳菇多糖提取工艺及抗氧化活性研究

Optimization of extraction process of polysaccharides from wild Lactarius volemus by response surface method and investigation of antioxidant activity

摘要

摘要: 本文意在优化多汁乳菇多糖的提取工艺,探讨多汁乳菇多糖抗氧化活性。本文以浸提温度、浸提时间、液料比为考察因素,在单因素实验的基础上,设计响应面法Central-Composite中心组合实验,对多汁乳菇多糖提取工艺参数进行优化,同时,通过O₂^-·、·OH、DPPH及ABTS自由基清除实验探究了多汁乳菇多糖的抗氧化活性。结果表明,多汁乳菇多糖的最佳提取工艺:浸提温度90℃,浸提时间4 h,液料比33∶1 m L/g,对应多糖得率为2.18%,产品中多糖纯度为55.40%,蛋白含量为11.37%,不含淀粉;多汁乳菇多糖对O₂^-·、·OH、DPPH及ABTS自由基清除活性的IC50值分别为:868.16、280.00、342.06、167.65μg/m L。可见,响应面法可有效拟合多汁乳菇多糖得率与浸提温度、浸提时间、液料比之间的关系,且多汁乳菇多糖具有较高的抗氧化活性。

Abstract: In order to study the extraction process of wild Lactarius volemus polysaccharide ( LVP) and its antioxidant activity, Central-Composite Design ( CCD) of response surface design ( RSD) was used to optimize the extract conditions of LVP based on single factor tests and O₂^-·, ·OH, DPPH, ABTS radicals scavenging activities were tested for antioxidant activity evaluation.The results showed that the optimal condition for extraction of LVP was as follows: temperature of 90 ℃, water bath time of 4 h, ratio of water to raw material of 33∶ 1 m L/g, corresponding yield of LVP was 2.18%, containing 55.40% LVP, 11.37% protein and no starch. The IC50 of O₂^-·, ·OH, ABTS and DPPH radicals scavenging activities of LVP were 868.16, 280.00, 342.06, 167.65 μg/m L respectively.All in all, response surface design is reliable for the study of relationship between yield of LVP and temperature, water bath time, ratio of water to raw material, furthermore, LVP shows a higher antioxidant activity in radical scavenging experiments.