Abstract: Ninety-six SPF Kunming mice with 18 ~ 22 g body weight were randomly divided into 8 groups to investigate the effect of compound probiotics-litchi polyphenol on antidiarrhea, growth performance and antioxidation of mice. Health group, model group, positive control group, Lactobacillus acidophilus group, Clostridium butyricum group, litchi polyphenol group, compound probiotics ( Lactobacillus acidophilus and Clostridium butyricum) group, compound probiotics-litchi polyphenol group were included in present study.From the 1th to 14 th day of the experimental period, the mice were administered for prevention and therapy purpose except for health group and model group, all weight and feed intake were measured regularly.At the 10 th day, the diarrhea model in mice was prepared by intragastrical administration of castor oil.At the 14 th day, the serum SOD, GSH-Px and CAT were assessed.Results showed that, weight change and diarrhea of model group were most obvious at the10 th day. The diarrhea inhibition effect of compound probiotics-litchi polyphenol group was best. Antioxidant indexes suggest that compound probiotics group and litchi polyphenol group were significantly higher than that of model group on SOD, GSH-Px and CAT ( p < 0.05) , compound probiotics-litchi polyphenol with the model group had higher difference on GSH-Px and CAT ( p < 0.05) .It is demonstrated that the compound probiotics-litchi polyphenol have obvious antidiarrhea and antioxidant effect in the castor oil diarrhea mice model.