Abstract: The sericin adsorption and desorption ability from simulation filature of six types of resins, AB-8, LX-1180, D101, DA201-C, DEAE-52 and 201 × 7 were compared and the best resin was selected. The optimal sericin separation parameters were determined by static and dynamic absorption experiment.The results showed that sericin adsorption capacity and recoverity of DEAE-52 ion exchange resin were much higher than other resins reached 1.573 mg/g and 95.07%, respectively.During the column separation, filature wastewater flowed with 5 BV/h into column, and then was eluted with 4 BV of p H2, 0.05 mol/L sodium chloride solution by 2 BV/h, then 1.7 ~ 3.0 BV elution fractions was collected, the desorption ratio was 98.76%. After column separation, the recoverd sericin concentrated 16.33 times of wastewater, and the total recovery rate and purity of collected sericin were 95.26% and 96.71%, respectively.