Abstract: In order to study on the growth rules of the goat and improve the mutton production, it was explored the main signaling pathways and the expression of key genes of longissimus muscle, triceps muscle and glutaeus in Inner Mongolian Cashmere goat.Three skeletal muscles of three cashmere goats were obtained after being slaughtered and total RNA was extracted to establish c DNA library.The Illumina high-throughput sequencing technique and bioinformatics were used to determine transcript abundances and characteristics.The establishment of mRNA level analysis platform by transcription for three skeletal muscles in Cashmere goat received 18.64 Gb of data, 92278723 reads of double end.The important genes belong to the myosin light chain and myosin heavy chain family.These results suggested that there were many differences in the muscle transcriptomes between these three skeletal muscles.