高效液相色谱法测定山楂叶中的9种酚类成分

Determination of nine phenolic components in the leaves of Chinese hawthorn by HPLC

摘要: 利用反相高效液相色谱法同时测定山楂叶中的9种酚类成分。结果表明,9种酚类成分在2~500μg/m L之间呈良好的线性关系,r介于0.9995~0.9999。加标回收率为93.7%~110.2%,相对标准偏差在0.69%~4.58%之间。对29个品种的山楂叶进行测定,9种酚类成分的平均含量由低到高依次为:异槲皮苷、金丝桃苷、原花青素C1、原花青素D1、表儿茶素、原花青素B2、绿原酸、红果酸、牡荆素鼠李糖苷。其中黄酮和酚酸含量较高,达15 mg/g D.W左右,原花青素类成分含量达6 mg/g D.W。该方法具有简便、准确的优点。

Abstract: Nine phenolic components in the leaves of Chinese hawthorn were determined by RP-HPLC simultaneously. The results indicated that the linearity ranges of nine compounds were between 2 ~ 500 μg/m L and the correlation coefficient ( r) were between 0.9995~0.9999.The recovery was 93.7% ~110.2%.The relative standard deviations were between 0.69% ~4.58%.The average content of nine phenolic components from low to high were isoquercitrin, hyperoside, procyanidin C1, procyanidins D1, epicatechin, proanthocyanidin B2, chlorogenic acid, eucomic acids, vitexin-2″-O-rhamnoside. Among them, the content of flavonoids and phenolic acids was higher than 15 mg/g D.W and the content of proanthocyanidins was up to 6 mg/g D.W.The method of HPLC was simple and accurate.