响应面法优化黑果枸杞中原花青素提取工艺
Optimization of extraction of proanthocyanidins from Lycium ruthenicum Murr.by response surface analysis
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摘要: 为了提高原花青素的得率,在单因素实验的基础上,采用响应面实验设计优化从黑果枸杞中提取原花青素的工艺。通过考察乙醇体积分数、提取温度、提取时间三个因素及其交互作用对原花青素得率的影响,建立了该工艺的二次多项数学模型。实验表明,曲面回归方程拟合性良好,乙醇体积分数、提取温度、提取时间对响应值均有极显著影响,在乙醇体积分数56%、提取温度46℃、提取时间48 min的条件下,黑果枸杞原花青素得率为4.315%,与响应面预测值相比,相对误差为1.5%,说明本研究方法是一种适合于从黑果枸杞中提取原花青素的方法。Abstract: In order to improve the yield, based on the single factor experiments, the response surface methodology was employed to optimize the extraction conditions of proanthocynidins from Lycium ruthenicum Murr.. The process of quadric polynomial mathematics model was established by investigating the effect of ethanol concentration, extraction temperature, extraction time and their interaction on proanthocyanidins.Experiments showed that regression equation fit well with experimental data and the effect of investigated parameters were extremely significant. The optimal of extraction conditions including ethanol concentration, extraction temperature, extraction time were 56%, 46 ℃ and 48 min respectively. The content of proanthocyanidins extracted from Lycium ruthenicum Murr.under the conditions was 4.315%.Compared with the predicted value of response surface, the error was 1.5%, which indicates that it is suitable for proanthocyanidins extracts from Lycium ruthenicum Murr.
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