竹荪中多酚提取工艺优化及其抗氧化活性研究
Study on the extraction and antioxidant activity of total phenolic compounds from Dictyophora indusiata
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摘要: 本文以长裙竹荪为原料,以多酚得率为考察指标,通过乙醇浓度、料液比、提取温度和提取时间4个单因素实验和正交实验确定竹荪多酚的最佳提取条件;并且通过竹荪提取液对2,2-二苯代苦味酰基（DPPH）自由基和羟基自由基的清除效果,评价竹荪提取液的体外抗氧化活性。结果表明:在乙醇浓度为40%、提取温度为80℃、料液比为1∶40 g/m L、提取时间为5 h的条件下,竹荪多酚的得率最高,可达（8.18±0.52）mg/g;竹荪提取液呈现出较好的抗氧化活性,且与浓度存在一定的量效关系,在质量浓度为20 mg/m L时,提取液对DPPH自由基的清除率可达84.28%±1.15%,对羟基自由基清除率达到76.49%±1.14%。Abstract: The contents of polyphenols from Dictyophora indusiata were determined by the Folin-Ciocalteu method in the paper.The effects of optimal alcohol concentration, liquid-solid ratio, extraction time and extraction temperature yield of D.indusiata were investigated, and the extraction technology was optimized by single-factor experiments and orthogonal design.The antioxidant activity of D.indusiata phenols was also studied.The results showed that the optimal extraction conditions were found that the optimal alcohol concentration, solid-liquid ratio, extraction time and extraction temperature were 40%, 1∶ 40 g/m L, 80 ℃ and 5 h respectively.Under these conditions, the largest extraction quantity of total phenols could reach ( 8.18± 0.52) mg/g.The DPPH radical scavenging ratio and ·OH radicals of the polyphenol extracts reached 84.28% ± 1.15% and76.49% ± 1.14% respectively when the extract concentration was 20 mg/m L.
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