Abstract: This study focused on the growth development and oil synthesis of Trichosporon cutaneum. The morphology of T.cutaneum, OD value, sugar and nitrogen concentrations, p H, oil content and the profile of the total fatty acid were individually measured and analyzed.The study demonstrated that the quantity of extracted oils from T. cutaneum was well agreed with its intracellular oils at 80 h, at this time the production and content of oil were 3.66 g/L and 39.20%, respectively. Meanwhile, results showed that the time from 0 h to 6 h was the lag phase, at which the consumptions of sugar and nitrogen were obvious, and the cell numbers was increased slowly.From 6 h to 25 h, the concentrations of sugar and nitrogen were rapidly decreased, and the cell numbers was largely increased, thus this period represented the logarithmic phase. From 25 h to 120 h, the consumption rate of sugar was higher than that of nitrogen, and the intracellular oils were gradually accumulated at this period, which named stationary phase. Thereafter, cells entered into decline phase, at which the concentrations of sugar and nitrogen were maintained constantly at low levels.More importantly, long chain saturated fatty acids were the most mainly fatty acid in total lipid of T.cutaneum, giving a new source for the preparation of biodiesel.