莲房原花青素低聚体微乳液的稳定性及抗氧化活性分析

陈娅; 尹浩; 李肖朋; 隋勇; 谢笔钧; 孙智达

doi:10.13386/j.issn1002-0306.2017.07.005

莲房原花青素低聚体微乳液的稳定性及抗氧化活性分析

Stability of micromulsion encapsulating oligomeric procyanidins from lotus seedpods and antioxidant activity of lsopc in micromulsion

摘要

摘要: 制备莲房原花青素低聚体（LSOPC）微乳液,通过测定微乳液粒径,研究温度、Na Cl、糖、金属离子等对LSOPC微乳液稳定性的影响。结果表明:LSOPC微乳液的粒径是（0.33±0.02）μm,在低温条件下比较稳定;低浓度Na Cl、含量低于9%的葡萄糖和蔗糖对LSOPC微乳液的稳定性无显著影响;一定浓度下,K+、Mg²⁺、Cu²⁺、Al³⁺对LSOPC微乳液稳定性无显著影响,Ca²⁺、Fe²⁺、Zn²⁺、Fe³⁺则显著影响其稳定性,粒径会有不同程度的增大。抗氧化活性实验结果表明,LSOPC微乳液和LSOPC溶液清除DPPH自由基IC50值分别为（6.55±0.04）、（7.33±0.04）μg/m L,前者的还原力强于后者,表明LSOPC微乳化后其抗氧化性显著增强。

Abstract: Microemulsion encapsulating oligomeric procyanidins from lotus seepods ( LSOPC) was fabricated. Mastersizer 2000 was used to evaluate the particle size. And the stability of LSOPC microemulsion was investigated. The particle size of LSOPC microemulsion was ( 0.33 ± 0.02) μm, and LSOPC microemulsion was stable under low temperature condition.Low concentration Na Cl, glucose and sucrose had little impact on the stability of LSOPC microemulsion. K+, Mg²⁺, Cu²⁺, Al³⁺ had less effect.Ca²⁺, Fe²⁺, Zn²⁺, Fe³⁺ were found to affect the stability of microemulsion, and the particle size of microemulsions increased to different extents.The IC50 values of free radical DPPH scavenging activity of LSOPC microemulsion and LSOPC solution were ( 6.55 ± 0.04) , ( 7.33 ± 0.04) μg/m L respectively.Reduction ability of LSOPC microemulsion was better than LSOPC solution.The results indicated that the antioxidant activity of LSOPC microemulsion was much better than LSOPC solution.