Abstract: The stability and anti-aging effect of oligomeric proanthocyanidin from Rhodiola rosea L. ( OPCRR) was studied.The results of stability showed that the OPCRR content would decrease under the condition of light, Fe3 +, Sn2 +, Al3 +, keep stable at low p H, low temperature, K+, Mg2 +, Na+and Cu2 +.The results of anti-aging effect showed that the concentration of 0.04%, 0.12% and 0.36% OPCRR had various degrees of prolonging effect on the average life and the highest life of Drosophila melanogaster, and the most effective dose was 0.12% OPCRR, in which the highest life and average life of Drosophila melanogaster was up to 72 d and 55 d respectively.In 0.12% and 0.36% OPCRR groups, the SOD activities were significantly increased ( p < 0.05) and MDA contents were significantly decreased ( p < 0.05) .The study provided the theoretical basis for deeply research and application of oligomeric proanthocyanidin from Rhodiola rosea L.in future.