响应面法优化红胞藻中藻红蛋白提取工艺及体外抗氧化性研究
Optimization of ultrasonic wave extraction technique of phycoerythrin from Rhodophyta salina by response surface analysis and its antioxidant activity
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摘要: 利用响应面分析法优化藻红蛋白的提取工艺。在单因素实验基础上,使用硫酸铵分级沉淀提取纯化藻红蛋白,选超声时间、温度、功率为影响因素,以藻红蛋白得率为响应值,根据Box-Behnken实验设计原理对藻红蛋白提取工艺进行响应面分析,优化藻红蛋白提取工艺。结果表明:在超声时间为16 min、温度为33℃、功率为750 W时藻红蛋白得率取得最大值。在此优化条件下进行验证性实验,测得蛋白得率为15.93%,与预测值相对误差为2.50%,表明该优化工艺具有良好的可行性。藻红蛋白对O-2·和ABTS+·具有一定的清除作用,其IC50分别为0.025 mg/m L和0.023 mg/m L。Abstract: Response surface methodology was utilized to optimize ultrasonic wave extraction technique of phycoerythrin from Rhodophyta salina. Based on single factor test,ammonium sulfate fractionation precipitation was used to purify phycoerythrin with the tempreture,extraction power and extraction time as influential factors. A three- variable,Box- Behnken experiment design combined with response surface analysis was used to optimize the extraction technique. And the yield of phycoerythrin was selected as the response value.Results indicated that the optimal extraction process conditions were determined as 16 min,33 ℃ and 750 W for yield of phycoerythrin.Under this conditions,the yield of phycoerythrin was 15.93% with a 2.50% error compared with the actual value. Phycoerythrin had the ability to clear off superoxide free adicals( IC_(50)= 0.025 mg / m L) and ABTS free radical( IC50= 0.023 mg / m L)
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