青藏高原部分地区牦牛与黄牛源沙门氏菌的分离鉴定与药敏实验
Study on the serovar and antibiotic resistance of yak and cattle Salmonella from some areas of Qinghai-Tibetan plateau
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摘要: 沙门氏菌(Salmonella)是一种重要的人畜共患病原菌。本研究采集了来自青藏高原部分地区的无腹泻症状的牦牛样品(194份)及黄牛样品(98份)共计292份,进行沙门氏菌株分离与鉴定并进行抗生素药敏实验。结果发现在牦牛样品中分离出15株沙门氏菌,其中粪便的检出率为9.23%,肌肉检出率为4.44%,肝脏检出率为11.86%,总检出率为7.73%,共5种血清型;在无腹泻症状的黄牛样品中分离出12株沙门氏菌,其中粪便的检出率为22.86%,肌肉检出率为2.70%,肝脏检出率为11.54%,总检出率为12.24%,共4种血清型。在药敏实验中,牦牛源的沙门氏菌总体耐药性小于黄牛源。但牦牛和黄牛源都对四环素、林可霉素、麦迪霉素等抗生素都呈现较高的耐药性。从实验结果可以得出,牦牛的沙门氏菌感染率和耐药性都低于黄牛。Abstract: Salmonella is an important zoonotic pathogens. This study collected 292 healthy yak and cattle samples in Sichuan Northwest plateau for Salmonella separation and of which antibiotic resistance research. The results showed that 15 plants Salmonella( 5 serovars) were isolated in yak which had no diarrhea symptoms samples,and the total detection rate was 7.73%,and the detection rate in fecal was 9.23%,muscle was 4.44%,liver was 11.86% respectively.12 plants Salmonella( 4 serovars)were isolated in cattle which had no diarrhea symptoms samples,and total detection rate was 12.24%,the detection rate in fecal was 22.86%,muscle was 2.70%,liver was 11.54% respectively. In the antibiotic susceptibility test,the resistance of yak Salmonella was less than cattle.However,yak and cattle Salmonella were showed high resistance to tetracycline,lincomycin and midecamycin.The yak Salmonella infection and antibiotic resistance were lower than cattle.
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