双水相萃取法分离鱼腥草总黄酮的研究

Optimization of aqueous two-phase extraction of total flavonoids from Houttuyniae cordata Thunb.

摘要: 利用聚乙二醇（PEG）-硫酸铵双水相体系对鱼腥草总黄酮进行萃取分离。通过单因素实验分别考察了PEG分子量、两相质量比、萃取温度、萃取p H对分离提取鱼腥草中总黄酮的影响,并用正交实验优化了萃取工艺。经优化后,鱼腥草总黄酮在以mPEG800∶m（NH4）2SO4=2.0∶2.0为双水相体系,萃取p H为3.0,水浴温度为50℃时,萃取率可高达98.96%。本方法简单高效,绿色环保,可为鱼腥草总黄酮的高效利用提供参考。

Abstract: To separate the total flavonoids from Houttuyniae cordata Thunb. by aueous two- phase systems composed of polyethylene glycol( PEG) and phosphate salts,an orthogonal experimental design method was applied to evaluate the influence of the average molecular mass of PEG,mass ratio of the two phases,temperature and p H value on total flavonoids extraction.The extraction rate was achieved 98.96% under the optimal conditions:mPEG400∶ m( NH4) 2SO4= 2.0 ∶ 2.0,temperature was 50 ℃,p H was 3.0. The result indicated that the aqueous two- phase extraction was simple,high quality and environment friendly,and also could apply some useful information for the efficient use of the total flavonoids in Houttuyniae cordata Thunb..