Abstract: Different enzyme- immobilized magnetic adsorbents were prepared by using glutaraldehyde to crosslink TG,Pepsin and Papain on amination magnetic microspheres. The adsorbents structure characteristics were analyzed by scanning electron microscope and infrared spectroscopy.The effect of initial concentration,adsorbent temperature,time and acidity on the adsorption of tetracycline in aqueous solution was investigated. Experimental results showed that the static adsorption accorded with the Pseudo- second- order kinetic and Freundlich adsorption isotherm,adsorption mechanism was not only multilayer chemical adsorption process but also endothermic process.Under the same adsorption temperature,the adsorption rate of order was Pepsin- AMM >Papain- AMM > TG- AMM. In the adsorption temperature was 318 K,adsorption process was spontaneous,the maximum adsorption capacities were 99.72,64.97 and 67.52 mg / g.