基于三元二次正交设计的文蛤水解肽制备工艺优化及ACE抑制活性分析

Enzyme hydrolysis preparation and ACE inhibitory activity of bioactive peptides from Meretrix lusoria

摘要: 本文以文蛤蛋白为原料,选用复合蛋白酶进行酶解并以水解度作为测定指标,在单因素实验基础上进行三元二次正交设计,对获得最优工艺参数进行验证,并对文蛤ACE抑制肽的活性及氨基酸组分进行分析。实验得出文蛤ACE抑制肽酶解最优工艺参数为底物浓度10%,酶解p H8.7,加酶量4.5%和酶解温度51℃,酶解时间3 h,此酶解工艺条件下对应的水解度为33%,酶解液离心所得上清液经HPLC测定其ACE抑制率为35%。

Abstract: Meretrix lusoria protein was hydrolyzed by protease enzyme through the ternary quadratic orthogonal design based on the single factor experiment,and bioactivity against angiotensin converting enzyme( ACE) of peptides was investigated. Optimal hydrolysis conditions were found that hydrolysis time was 3 h,the substrate concentration was 10%,the value of p H was 8.7,the addition of enzyme was 3% and the hydrolysis temperature was 51 ℃. The degree of hydrolysis was 33%. In vitro ACE inhibitory activity of bioactive peptides from Meretrix lusoria was performed by high performance liquid chromatography.The results suggested that the activity against the ACE of bioactive peptides was 35%.