Abstract: To optimize the ethanol extraction of total flavonoids from Zizyphus jujube cv. Huizao,the effects of extraction times of ultrasonic,temperature,liquid to solid ratio,the volume fraction of ethanol and their interaction on the extraction yield of jujube flavonoids were studied with Box- Behnken,on the basis of single factor tests. The optimum extraction conditions were obtained as follows: extraction time 30 min,extraction temperature 40 ℃,the ratio of solid to liquid 1∶ 29( g∶ m L),the volume fraction of ethanol 70%.Under the optimal conditions,the extraction rate of total flavonoids from Zizyphus jujube cv.huizao was 3.266 mg / g,which RSD for 0.38% agreed well with the predictive value.The optimized conditions for flavonoids extraction from Zizyphus jujube cv.Huizao were reliable.