离子交换色谱法测定10种热带水果中的葡萄糖、蔗糖和果糖

于璐; 周光明; 沈洁; 余艳丽

doi:10.13386/j.issn1002-0306.2016.22.010

离子交换色谱法测定10种热带水果中的葡萄糖、蔗糖和果糖

Determination of glucose,sucrose and fructose in 10 kinds of tropical fruits by ion chromatography

摘要

摘要: 建立了高效阴离子交换-脉冲安培检测（High performance anion exchange-pulsed amperometric detection,HPAE-PAD）测定火龙果、莲雾、牛油果等10种热带水果中葡萄糖、蔗糖和果糖的检测方法。热带水果中的多糖经超声萃取后,用METROSEP CARB 1（150 mm×4.0 mm）色谱柱进行分离,以30.0 mmol/L Na OH为流动相,等度洗脱,用安培检测器检测,18 min可完成对样品多糖的分离和定量分析。经测定,葡萄糖和蔗糖的检出限分别为0.1209μg/m L和0.2827μg/m L,线性范围为1.0⁷0.0μg/m L;果糖的检出限为0.5501μg/m L,线性范围为5.0⁸0.0μg/m L。样品溶液连续5次进样,3种糖的相对标准偏差为0.67%⁷.04%,平均回收率为78.70%¹17.75%。该方法前处理简单、选择性好、灵敏度高,可用于热带水果中可溶性糖的测定。

Abstract: A high performance anion exchange- pulsed amperometric detection chromatographic method was applied to determine glucose,sucrose and fructose in pitaya,wax apple,avocado and other 7 kinds of tropical fruits. After ultrasonic extraction,the polysaccharide in tropical fruits was separated by METROSEP CARB 1( 150 mm × 4.0 mm) anion exchange column and detected by pulsed amperometric detector.30.0 mmol/L Na OH was used for isocratic elution.Analysis of the samples could be finished within 18 min.Detection limit of glucose and sucrose were 0.1209 μg / m L and 0.2827 μg / m L respectively. The linearity ranged from 1.0 μg / m L to 70.0 μg / m L.Detection limit of the fructose was 0.5501 μg / m L. The linearity range was 5.0 ~ 80.0 μg / m L. The RSDs of 3polysaccharide contents for 5 consecutive determinations were between 0.67% and 7.04%,and the spike recoveries were between 78.70% and 117.75%. The method had the characteristics of simple pretreatment,good selectivity and high sensitivity,and it could be applied in the determination of soluble sugars in tropical fruits.