Abstract: A modified Qu ECh ERS method followed by liquid chromatography- tandem mass spectrometric analysis was developed for the determination of biogenic amines in aquatic products. The samples was diluted with acetonitrile / methanol solution( 9 /1) and cleaned up with primary secondary amine( PSA) and Octadecylsilane( C18) sorbent.The separation of biogenic amines was performed by hydrophilic interaction liquid chromatography( HILIC) column and gradiently eluting with acetonitrile/formic acid- water( 1%) without derivatization. Biogenic amines were analyzed by positive electrosprary ionization( ESI~+) tandem mass spectrometry under multiple reaction monitoring( MRM) mode.Quantification was achieved using external standard method.The linearities were excellent in the range of 10~200 ng / m L.The limits of quantification( LOQ,S / N = 10) were 0.10 mg / kg.The recoveries of biogenic amines spiked in matrices were between 74.18% and 90.15% at the spiked levels of 0.10 mg / kg. The relative standard deviations( RSDs,n = 6) were in the range of 2.22% ~8.04%.The method( quick,easy,safe) could meet the requirements of the daily work for the determination of biogenic amines in aquatic products.