Abstract: EST-SSR is a new molecule marking technology to exploit Simple Sequence Repeats( SSR) based on Express Sequence Tags( EST).It was widely used in the study of crop genomics because of its good transferability in different crops. The transferability of EST-SSR in different grain crops could cut the costs of primers development and improve efficiency.It could lay the foundation of the crops that genetic studies are weak. ESTSSR makers and its transferability in different grain crops compared with G-SSR were introduced in this paper.The disadvantages and improvements in order to accelerate the development of crop genomics by using the high transferability of EST-SSR were reviewed.