Abstract: In this research pectin-chitosan layer by layer modified liposomes were successfully prepared. The effects of main factors on the encapsulation efficiency and turbidity were evaluated,and optimal formula was obtained by orthogonal experiments using encapsulation efficiency as the main index.The results indicated that the volume ratio and concentration ratio of pectin solution to chitosan solution had significant effects on the encapsulation efficiency and turbidity of pectin-chitosan layer by layer modified liposomes. The process of modification could diminish the properties differences affected by the mass ratio of lecithin to cholesterol. The optimal formula was the volume ratio of pectin solution to chitosan solution 2 ∶ 1,concentrations of pectin solution and chitosan solution 0.2% ∶ 0.2%,the mass ratio of lecithin to cholesterol 5∶ 1,addition amount of lycopene 2.5 mg.The encapsulation efficiency of the optimized pectin-chitosan modified liposomes was significantly higher than that of conventional liposomes,which was 97.8% and 85.4% for them,respectively,the particle size distribution of the obtained pectin-chitosan modified liposomes was narrow with the mean particle size of 1870.4 nm and polydispersity index of 0.231.