榆干离褶伞溶栓酶对血栓模型大鼠血小板活性的影响

刘雪; 王玉娇; 全吉淑; 沈明花

doi:10.13386/j.issn1002-0306.2016.20.060

榆干离褶伞溶栓酶对血栓模型大鼠血小板活性的影响

Effect of fibrinolytic enzyme from Lyophyllum ulmarium on platelet activation in thrombotic rat

摘要

摘要: 目的:研究榆干离褶伞（Lyophyllum ulmarium,L.u）溶栓酶的抗血栓作用及对血小板活性的影响。方法:三氯化铁诱导大鼠颈动脉血栓模型,检测血栓重量,用流式细胞仪检测CD62P的表达,酶联免疫吸附法测定p-选择素、血管性血友病因子（v WF）、血小板膜糖蛋白Ⅱb/Ⅲa、血栓素B2（TXB2）和6-酮-前列腺素E1α（6-keto-PGE1α）的水平。结果:与模型组相比,L.u溶栓酶处理以后大鼠血栓重量明显减轻,CD62P阳性率、p-选择素、v WF、血小板膜糖蛋白Ⅱb/Ⅲa以及TXB2的含量不同程度地下降,对6-keto-PGE1α的影响不显著。结论:L.u溶栓酶具有抗血栓作用,其机制可能与抑制血小板的活化作用有关。

Abstract: Objective: To study the antithrombotic effect and the effect on platelet activity of fibrinolytic enzyme from Lyophyllum ulmarium.Methods: The carotid arterial thrombosis model was induced by ferric chloride in rats,and then the thrombus weight was detected.The expression of CD62 p on the platelet were applied by flow cytometric analysis,and the levels of p- selectin,v WF,platelet glycoprotein Ⅱb / Ⅲa( GPⅡb / Ⅲa),TXB2 and 6- keto- PGE1α,in plasma were measured.Results: Compared with the model group,fibrinolytic enzyme from Lyophyllum ulmarium reduced thrombus weight,suppressed the expression of CD62 p,and reduced the contents of P- selectin,v WF,platelet glycoprotein GPⅡb / Ⅲa and TXB2.However,there was no significant difference in the 6- keto- PGE1αlevel.Conclusion: Fibrinolytic enzyme from Lyophyllum ulmarium had an antithrombotic activity,and the mechanism might be due to inhibition of platelet activity.