甘露糖对免疫性肝损伤小鼠的保护作用
Hepatoprotective effects of mannose on immunological liver injury in mice
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摘要: 目的:研究甘露糖对卡介苗与脂多糖（Bacillus Calmette-Guérin,BCG/lipopolysaccharides,LPS）诱导的小鼠免疫性肝损伤的保护作用及作用机制。方法:将小鼠随机分成正常组、模型组、甘露糖低剂量组（20 mg/kg）、甘露糖高剂量组（100 mg/kg）,采取BCG联合LPS诱导小鼠肝损伤。取肝脏、脾脏、胸腺,测定脏器指数。用试剂盒的方法分别检测各组小鼠血清中谷丙转氨酶（alanine aminotransferase,ALT）、谷草转氨酶（aspartate transaminase,AST）活力,丙二醛（malonic dialdehyde,MDA）、一氧化氮（NO）、肿瘤坏死因子（tumor necrosis factor,TNF-α）、细胞白介素（interleukin-β,IL-β）含量及超氧化物歧化酶（superoxide dismutase,SOD）与过氧化氢酶（catalase,CAT）活力,并取肝组织病理切片观察。结果:与模型组相比,甘露糖高剂量组脾脏指数和胸腺指数显著降低（p<0.05）,且血清中ALT、AST活性和MDA、NO、TNF-α、IL-β含量显著下降（p<0.05）,同时SOD和CAT活性显著升高（p<0.05）;甘露糖低剂量组有一定好转,但基本未达显著水平。结论:甘露糖对BCG/LPS诱导的小鼠免疫性肝损伤有很好的保护作用。Abstract: Objective: The hepatoprotective effects of mannose and possible mechanism were researched on BCG /LPS- induced immunological liver injury in mice.Method: Mice were divided into four groups,namely control,model and two mannose- treated groups.BCG and LPS were used to induce the immunity hepatic injury model of mice in the experiment.Blood,liver and organs were sampled for detection of biochemical and immunological index,and the pathological changes of liver.Results: Compared with the model group,high dose of mannose makes spleen index and thymus index significantly decreased( p < 0.05),and in serum the activities of ALT/ AST significantly have been reduced.Meantime,content of MDA / NO / TNF-α / IL-β are also significantly lower( p < 0.05),while SOD and CAT activities observably increase( p < 0.05). Low dose of mannose had some improvement,but it was not significant.Conclusion: Mannose had hepatoprotective effects against BCG / LPS- induced liver damage in mice.
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