响应面法优化超声波辅助提取玫瑰香葡萄中芪化物的工艺条件

Optimization of extraction process of stilbenes from Muscat Grape by ultrasonic method of response surface experiments

摘要: 以玫瑰香葡萄为原料,采用超声波辅助提取工艺,对其中的芪化物提取条件进行优化。在单因素实验基础上,以白藜芦醇、白藜芦醇苷、紫檀芪化物提取量综合指标为响应值,通过响应面法得到最佳工艺条件。结果表明以乙酸乙酯为提取液,在提取温度57℃、提取时间32 min、料液比1∶15 g·m L-1条件下,玫瑰香葡萄中芪化物提取量理论值为105.30μg/g,实际提取量可达到104.97μg/g,相对误差为0.31%。最佳工艺条件可以应用于玫瑰香葡萄中芪化物的提取。

Abstract: Muscat Grape wes used as the material to extract stilbenes through ultrasonic method.Combined with the single factor test results,response surface methodology( RSM) was applied to optimize the extraction conditions.And the total content of Resveratrol,Piceid and Pterostilbene was used as the investigation index.A maximum extraction rate was achieved.The optimal extraction conditions were used Ethyl acetate as solvent,temperature of57 ℃,extraction time of 32 min,and solid to liquid ratio 1 ∶ 15 g·m L- 1. Under the optimal extraction conditions,the predicted value of stilbenes in Muscat Grape was 105.30 μg / g,and the actual value was 104.97 μg / g.Moreover,the relative error of the established model for extraction efficiency was 0.31% compared with the predicted value.The maximum extraction rate was appropriate for the extraction of stilbenes in Muscat Grape.