超声辅助提取雨生红球藻渣多糖工艺优化
Optimization of process in extracting polysaccharide assisted by ultrasonic wave from Haematococcus pluvialis residues
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摘要: 以提取过虾青素后的雨生红球藻渣为原料,采用超声辅助热水浸提法提取藻多糖。在单因素实验基础上,通过正交实验设计对雨生红球藻多糖提取工艺进行优化,并与传统的热水浸提工艺进行对比。结果表明,雨生红球藻多糖的最佳提取工艺为:超声功率400 W,超声时间30 min,水浴温度90℃,水浴时间3 h,料液比1∶25,在此条件下多糖得率为3.48%,得率比传统法提高了27%。超声辅助热水浸提法是雨生红球藻多糖提取的有效途径,为藻多糖的进一步研究提供基础,为雨生红球藻渣的综合开发利用提供理论依据。Abstract: Polysaccharide was extracted from Haematococcus pluvialis residue after extraction of astaxanthin by ultrasonic treatment cooperated with hot water extraction. Operating parameters for hot water extraction of polysaccharide from Haematococcus pluvialis were optimized using an orthogonal array design. The best results were obtained after ultrasonic treatment at 400 W for 30 min at a solid- to- solvent ratio of 1∶ 25( g / m L),water bath temperature of 90 ℃ for 3 h.Under such conditions,the extraction yield of polysaccharides was 3.48%,which was approximately 27% higher than that obtained by hot water extraction alone. The results showed that ultrasonic treatment cooperated with hot water extraction was a potential effective way,and providing theoretical basis for in depth research of Haematococcus pluvialis polysaccharide and for the development and application of residue.
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